Fluorescent probes for lipid rafts
WebJul 1, 2011 · BODIPY-GM 1 can be used as a marker of lipid rafts in cells Fluorescence emission of the labeled ganglioside BODIPY-GM 1 as a function of its density on the membrane surface behaved in the manner expected for BODIPY. Probe density was varied by forming mixed micelles that were composed of BODIPY-GM 1 and GM 1 at different … WebMar 26, 2007 · Lipid rafts can be visualized by two-photon microscopy by using C-laurdan as a probe. Our results show that the lipid rafts cover 38 % of the cell surface. Publication types Research Support, Non-U.S. Gov't MeSH terms 2-Naphthylamine / analogs & derivatives* 2-Naphthylamine / chemistry Calibration Cell Line, Tumor Cell Membrane / …
Fluorescent probes for lipid rafts
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Web1 day ago · Further, costaining assay showed that the fluorescence of RCHC1-COOH overlaps very well with that of commercial lipid raft probe CLS-PEG-FITC, indicating that the dye specially targets lipid rafts (cholesterol- and sphingolipid-enriched microdomains) within plasma membrane. Web52 rows · Nov 8, 2024 · There are three major types of lipid probes: fluorescent probes can be used for identifying target membranes or lipids [1,2,3,4], spin-probes are useful …
WebLipid Phase Behavior and Stabilization of Domains in Membranes of Platelets WebLaurdan is a polarity sensitive fluorescent probe for imaging lipid rafts in membranes (also known as lipid microdomains). It is suitable for use with generalized polarization (GP) imaging and scanning fluorescence correlation spectroscopy (FCS).
WebMar 13, 2007 · Solubility and sensitivity. C-laurdan, a membrane probe, shows higher sensitivity to membrane polarity and emits stronger two-photon excited fluorescence than … WebFeb 4, 2013 · Fluorescence microscopy is the tool-of-choice to investigate membrane lipid domains [12], [13] as it can equally be applied to model and cell membranes and avoids fixation, which can introduce artifacts in lipid and protein organization [14].
WebApr 2, 2008 · Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. onslow actorWebThe fluorescence spectrum of the probe laurdan, which incorporates into the lipid phase in the membrane, is correlated to its physical state. Two-photon infrared excitation techniques have been successfully applied to detect laurdan emission. onslow accommodation western australiaWebPralle, A., Keller, P., Florin, E. L., Simons, K., and Horber, J. K. (2000) Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells. J. Cell Biol.148, 997–1008. Bergelson, L. D., Molotkovsky, J. G., and Manevich, Y M. (1985) Lipid-specific fluorescent-probes in studies of biological-membranes. Chem. Phys. onslow adult protective servicesWebSep 29, 2009 · Lipid packing can be determined with fluorescent membrane probes such as 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan) ( 28 – 30 ). When integrated into the bilayer, the wavelength of the emission peak of the dye is dependent on the presence of water in the membrane. iodized breadWebApr 1, 2004 · Furthermore, a green fluorescent protein-D4 fusion protein (GFP-D4) was revealed to be useful for real-time monitoring of cholesterol in lipid rafts in the plasma membrane. In addition, the expression of GFP-D4 in the cytoplasm might allow the investigations of intracellular trafficking of lipid rafts. iodized lecithin tabletsWebDec 18, 2013 · There are numerous fluorescent probes (majority of them are fluorescent dyes) which were successfully used to selectively detect the lipid rafts or the lipid … onslow airbnbWebFeb 11, 2011 · Finding lipid rafts: We report a two-photon turn-on probe (SL2) that emits strong two-photon excited fluorescence (TPEF) in lipid rafts and can detect the lipid rafts in live cells... iodized lecithin