Eea1 molecular weight

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WebBackbone manufacturer Clontech Backbone size w/o insert (bp) 4700 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name RAB7 (Homo … WebEEA1 (E-8): sc-365652 ... Molecular Weight of EEA1: 162 kDa. STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. DATA SELECT PRODUCT CITATIONS 1. Huang, S.H., et al. 2013. Epidermal growth factor receptor-containing

EEA1, an Early Endosome-Associated Protein. - Journal of …

WebEEA1 Antibody (PA1-063A) in ICC/IF. Immunofluorescent analysis of Early Endosomal Antigen 1 was performed using 70% confluent log phase … WebThis Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1. Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells. the sopranos from where to eternity

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WebMar 30, 2024 · Even though the depletion of VPS35 increased the size of the EEA1-labeled early endosomes, in line with SNX1 pattern, the endosomal actin patches were intact in the VPS35 KO cell line. ... (Molecular Probes, 1 μL per 22 × 22 mm coverslip) was added to the secondary antibody step. All coverslips were then mounted onto Mowiol supplemented … WebJun 2, 1995 · Membranes were incubated with affinity-purified human (Aff. pur. EEA1 Ab) or rabbit (Aff. pur. rabbit Ab) anti-EEA1 antibody ... although post-translational modification of EEA1 may contribute to the difference between its observed and predicted molecular weight. Rabbit Antiserum to Recombinant Protein Reacts with Cytoplasmic Vesicles and … WebEarly endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. the sopranos free streams

The p97 ATPase associates with EEA1 to regulate the size …

EEA1 Polyclonal Antibody (PA1-063A) - Thermo Fisher …

WebCell Signaling Technology Inc wo2 mouse monoclonal Wo2 Mouse Monoclonal, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. the sopranos full episodesWebMar 21, 2024 · Protein details for EEA1 Gene (UniProtKB/Swiss-Prot) Protein Symbol: Q15075-EEA1_HUMAN Recommended name: Early endosome antigen 1 Protein Accession: Q15075 Secondary Accessions: Q14221 Protein attributes for EEA1 Gene Size: 1411 amino acids Molecular mass: 162466 Da Protein existence level: PE1 NEW … the sopranos full cast and crew

"WebEEA1 (E-8) is available conjugated to agarose (sc-365652 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-365652 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either … " - Eea1 molecular weight

Eea1 molecular weight

EEA1 Antibody (NBP1-30914): Novus Biologicals

WebEEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy … WebEarly endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane …

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WebJun 2, 1995 · We have cloned a human cDNA encoding an evolutionarily conserved 180-kDa protein on early endosomes named EEA1 (Early Endosome Antigen1). EEA1 is associated with early endosomes since it co-localizes by immunofluorescence with the transferrin receptor and with Rab5 but not with Rab7. Immunoelectron microscopy… WebEEA1 Protein Molecular Weight & PI Early endosome antigen 1 (Endosome-associated protein p162) (Zinc finger FYVE domain-containing protein 2) Homo sapiens (Human). …

WebMar 1, 1999 · Abstract EEA1, an early-endosomal protein originally identified as an autoantigen, is essential for endocytic membrane fusion. It interacts with early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of the small GTPase Rab5. WebMay 1, 2024 · Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. …

WebMay 10, 2011 · The EEA1-containing high-molecular-weight complexes might constitute both EEA1 homodimer as well as EEA1 heterooligomers that contained other EEA1-interacting proteins. WebEarly endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane …

WebApr 10, 2024 · The lower molecular weight protein band, detected by the anti-human Alix antibody in bovine and swine cell lysates, may represent unspecific staining. ... like the early endosome-associated protein 1 (EEA1), recycling endosome-decorating Rab11, the late endosome marker Rab7, and the endolysosomal LAMP1 (lysosomal-associated …

WebFeb 4, 2000 · Early endosome antigen 1 (EEA1) is 170-kDa polypeptide required for endosome fusion. EEA1 binds to both phosphtidylinositol 3-phosphate (PtdIns3P) and to Rab5-GTP in vitro, but the functional role of this dual interaction at the endosomal membrane is unclear. the sopranos free watchWebMar 16, 2011 · CT subunit B Alexa Fluor ®-594 conjugate (CTxB-594) (Molecular Probes) was added to the wells at a final concentration of 1 µg/mL. After 1 or 3 h of toxin treatment the medium was removed and the coverslips were fixed with 3.7% formaldehyde in DMEM 10% BGS for 20 min. Coverslips were washed 3 times with 1× PBS and mounted onto a … myrtle beach niWebOct 13, 2024 · EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Because of the presence of complex endosomal … the sopranos full episodes 123WebEEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking ... Gene Name EEA1(Human) Molecular Weight 36.630kDa inclusive of tags; Add to Compare List. Creative BioMart. Phone: 1-516-855-7709. Email: [email protected]. myrtle beach nissan dealershipWebImmunogen: Human EEA1 aa. 3-281 Application: Immunofluorescence (Routinely Tested), Immunohistochemistry, Immunoprecipitation (Not Recommended) Target Molecular … myrtle beach nightlife reviewsWebEEA1 antibody can be used for detection of EEA1 by western blot at 0.06 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL 1: Cat. No. 1309 - Human Placenta Lysate PREDICTED MOLECULAR WEIGHT: 162 kDa Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. myrtle beach nike outletWebLane 1 - 2 : EEA1 antibody - Early Endosome Marker (ab2900) at 1/500 dilution, HEK293 Whole Cell lysate at 20 ug Lane 1 : as above Lane 2 : EEA1 peptide at 1 ug Secondary … the sopranos for free