WebBeef heart aconitase has been studied by low temper- ature magnetic circular dichroism (MCD) spectroscopy in the wavelength region 300 to I900 nm. Together with parallel electron paramagnetic resonance and ac- tivity measurements, these data enable correlations between Fe-S cluster-type and enzymic activity in aconitase. WebJan 1, 2024 · Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease. ...
Determination of Aconitase Activity: A Substrate of the
WebAconitase 2 ( ACO2 ) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been initially associated with infantile cerebellar-retinal degeneration combining optic atrophy, retinal degeneration, severe encephalopathy, epilepsy, and cerebellar ataxia1–3; … Weblevels of aconitase, whereas its overexpression decreases its levels and activity [15, 25] (Fig. 1). This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. … graphic packaging help desk
Reduced Expression of Aconitase Results in an Enhanced Rate of ...
WebThe osmotic and activity coefficients of aqueous mixtures of NaCl and MgCl/sub 2/ have been determined at 25/sup 0/C by using the isopiestic method. These measurements extend from low concentrations to the crystallization limits of the mixtures. They are critically compared to published isopiestic, direct vapor pressure, and emf data for this system. WebOct 1, 1974 · TAA is also known to play a role in the TCA cycle where it inhibits the aconitase (ACO), the enzyme converting citrate to isocitrate via cis-aconitic acid (CAA) intermediate [34] [35] [36 ... WebSep 17, 2024 · These intermediates are capable of oxidizing the cluster, which leads to iron release and consequent loss of the catalytic activity of aconitase. As the reaction of the Fe-S cluster with O 2 •-is fast (∼10 7 M-1 s-1), quite specific, and reversible in vivo, quantification of active aconitase has been used to evaluate O 2 •-formation in cells. chiropractic allergy