Cre reporter tool strain
WebPlasmid Cre Reporter from Dr. Niels Geijsen's lab contains the insert LoxP-DsRED-STOP-LoxP-eGFP and is published in Cell. 2015 Apr 23;161(3):674-690. doi: 10.1016/j.cell.2015.03.028. This plasmid is … Webvivo functionality of Cre-expressing mice, we have engineered an improved reporter strain for monitoring Cre-mediated excisions. The b-galactosidase-neomycin …
Cre reporter tool strain
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WebSep 21, 2024 · A Cre reporter tool strain can be also designed to have a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven fluorescent protein variant, inserted into the Gt (ROSA) 26Sor locus. Robust cell fluorescence is achieved following Cre-mediated recombination, thus allowing cell tracking. WebCre offers several levels of control over recombination: both the insertion site of Cre and the recombination events it triggers can be precisely targeted; and it can be inserted in the …
WebNational Center for Biotechnology Information WebThe R26R mouse strain should be of wide use for monitoring Cre expression, as well as for analysing cell lineages during development, and is available from the Induced Mutant Resource of the ...
WebResults. Throughout this study, we utilize the Ai14D reporter mouse strain (Madisen et al., 2010; Kauffman et al., 2024) to assay Cre activity throughout the body (Figure 1A).This reporter carries a transgene at the ‘safe harbor’ Rosa26 locus, with a ubiquitously expressed CAG promoter driving expression of a transgene with a 3× STOP cassette … WebThe JAX Cre Repository uses a lacZ Cre-reporter strain, B6.129S4-Gt(ROSA)26Sor tm1Sor/ J, to assess cre activity. β-Galactosidase staining of 10.5 dpc embryos is done … For Cre characterization work at the Jackson Laboratory, there is a 7‐day … The Jackson Laboratory Cre Repository uses whole-mount β-galactosidase … The Knockout Mouse Project (KOMP) is providing critical tools for understanding … Each new mutant strain is characterized genetically, genomically and …
WebMar 9, 2024 · A blue light-sensitive ACR2 has recently been used in several in vivo studies; however, the reporter mouse strain expressing ACR2 has not yet been reported. Here, …
WebAug 7, 2024 · So, the USF Health researchers created a knockin mouse incorporating a powerful genetic approach that could be used in future experiments. They made a mouse that expresses the bacterial enzyme … rpc sample submission formWeb007914 B6.Cg- Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze /J Ai14 is a Cre reporter tool strain designed to have a loxP -flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent … rpc server is unavailable windows 2016WebSep 27, 2024 · The mu lti-reporter mouse line for loci-of-recombination ( X) (MuX) will be useful to characterize recombination characteristics of mice expressing either Cre, Flp, Dre or Vika recombinase and... rpc runtime libraryWebApr 10, 2024 · The Dmp1-Cre mouse, expressing Cre from a 14-kb DNA fragment of the mouse Dmp1 gene, has become a common tool for studying gene function in osteocytes, but the presumed cell specificity is yet to ... rpc server is unavailable redditWebJan 15, 2009 · Crossing these mice with a Cre reporter strain showed that Cre DNA-mediated recombination was ubiquitously and effectively induced during various prenatal developmental windows. Background Cre recombinase expression levels were observed in some tissues in the absence of the inducer, mostly during late embryonic developmental … rpc saxophone mouthpieceWebHere, we describe a reporter system that is based on ligand-induced DNA recombination to express the reporter gene. This system converts a transient activation of a signal … rpc server is unavailable windows 2019WebAug 1, 2024 · The Cre reporter cassette (5.3 kb), which contained two loxP sites, flanking the eGFP coding sequence with a triple repeat of poly (A) elements directed by a cytomegalovirus early enhancer element and … rpc server is not available windows 10